To investigate the phytochemical screening and antibacterial activity of pet. Synthesis of silver nanoparticles using tridax procumbens and. Antibacterial activity of different parts of tridax procumbens against. In this research the synthesis of silver nanoparticles using plant tridax procumbens has been investigated. Previous researches have shown that the plant has antimicrobial activities and pharmacological effects. Methanol extract of tridax procumbens showed maximum antifungal activity. Antimicrobial activity of aqueous extracts of two plants were investigated by agar disc welldiffusion method against bacterial pathogens staphylococcus aureus, bacillus subtilus, escherichia coli, k. Pdf phytochemical screening and in vitro antimicrobial. India is gifted with varieties of large number of medicinal herbs because of variety of climatic conditions and seasons favorable for growth of many species of. The polyphenol and flavonoids are used for the treatment of various diseases triggered by oxidative stress. The present work is concerned with exploring antimicrobial activity of. The extracts of tridax procumbens have been reported to have various pharmacological effects, antimicrobial activity, wound healing property immunomodulatoryand effect on experimental animals.
Tridax procumbens have been used as indigenous medicine for a variety of ailments. Plant part pharmacological activity reference whole plant antimicrobial activity against both grampositive and gramnegative bacteria r. Assessment of antibacterial and antifungal activities of silver nanoparticles obtained from the callus extracts stem and leaf of tridax procumbens l. British journal of pharmaceutical research 14, 164173. In the present study, it was planned to investigate the phytochemical and in vitro antioxidant activity of the ethanol, methanol and aqueous extracts of tridax procumbens leaves. Pseudomonas aeruginosa, while the petroleum ether extract did not shown antibacterial activity. Biogenic synthesis of fe3o4 nanoparticles using tridax leaf. Antibacterial activity of tridax procumbens with special reference to nosocomial pathogens. Antimicrobial activity of biogenic silver nanoparticles. The order of susceptibility of tridax procumbens on. Invitro antibacterial, antioxidant potentials and cytotoxic.
In this present study focused on bacterial cellulose implementation with this plant. Muller hinton broth mh extract of mimosa pudica linn and tridax procumbens and nutrient broth nb media himedia pvt. Antioxidant, antidiabetic, antimicrobial and hemolytic activity of tridax procumbens r. Silver nanoparticles agnps were synthesized by reducing 2 mm solution of agno3 using leaf extract of tridax procumbens. The antimicrobial activity is greater in crude extracts than coloumn extracts table 2. Asian journal of research in biological and pharmaceutical sciences. Save nature to survive phytochemical screening and. The mean of three replicates of zone of inhibition mm around well with tridax procumbens mediated. Antibacterial activity of methanolic extract of tridax procumbens l. Antimicrobial activity whole plant of tridax has reported for its antimicrobial activity on various species of bacteria. Tridax procumbens plant has been used in traditional medicine to cure several ailments.
Green synthesis of silver nanoparticles using tridax. The results are compared with standard antibiotic streptomycin. Pdf phytochemical and antibacterial studies of leaves of. Flavonoids isolated from tridax procumbens tpf inhibit. Evaluation of phytochemical and antioxidant activity of. Phytochemical and pharmacological screening of combined. Antibacterial activity of tridaxprocumbens with special reference to nosocomial pathogens. The leaf juice possesses antiseptic, insecticidal and parasiticidal properties and is used. Sterols, tribulus terrestris, sida acuta, tridax procumbens introduction medicinal plants are essential part of human medicine, since the dawn of civilization and are the backbone of traditional medicine system in india 1. Anti microbial activity antimicrobial activity was explored by using acetone and methanolic extracts of plant parts as root, leaves and flowers respectively. Antibacterial activity was investigated by disc and agarwell diffusion method.
The antimicrobial activity of tridax procumbens performed by using agarwell diffusion method showed a result showcasing an effective limit when as opposed to pseudomonas vulgarisfor ethyl alcohol being used as solvent for extract. Pdf antibacterial activity of tridax procumbens with special. It also proves the traditional claim with regard to. Key words tridax procumbens, methonolic and ethyl acetate extract, antibacterial activity. Chitra pai 1, ujjwala kulkarni 2, manjusha borde 2, sowmya murali 3, p. Pdf antibacterial activity of tridax procumbens linn researchgate. January antimicrobial activity of tridax procumbens. Phytochemical estimation and antioxidant activity were carried out for aqueous. Synthesized agnps were characterized by uv spectroscopy, ftiratr, sem, and tem analyses. R engineering college, sivakasi626140, tamil nadu abstract tridax procumbens, is a weed extensively used in traditional medicine as anticoagulant. Mechanism of antibacterial action of the alcoholic extracts. The medicinal properties of different parts of tridax procumbens are listed in table 1 and phytochemical constituents of tridax procumbens plants are listed in table 2.
They represent rich source of antimicrobial agents 2. Besides, antioxidant activity of ethyl acetate extract ic. Plant biotechnology lab 1, department of botany, university of rajasthan, jaipur302044, rajasthan, india. Phytochemical and antibacterial studies of leaves of.
Pdf antibacterial activity of tridax procumbens linn. The extracts were stored in desiccators for preliminary phytochemical analysis and further testing of antimicrobial activity. Jmphtr 2 2014 1622 issn 20531826 invitro evaluation of the synergistic antimicrobial activities of zingiber officinale rosc and tridax procumbens linn against selected pathogenic bacteria a. In the antibacterial activity assay, different extracts of t. Herbs are natural remedies for the disease with higher safety profile and efficacy. Philomenas degree college, mysore, karnataka abstract. Antibacterial activity of roots extracts from tridax. The tridax procumbens flavonoids tpf, are well known for their medicinal properties among local natives. Tridax family compositae commonly known as ghamra and in english popularly called coat buttons because of appearance of flowers has been extensively used in ayurvadic system of medicine for various ailments and is. Previous finding revealed antibacterial activity of t.
Many reports has been given for the same pathogenic organisms28. Antibacterial evaluation of aqueous and methanol extracts of tridax procumbens flower on selected gram negative and grampositive bacteria. Antimicrobial activity preliminary phytochemical screening of ethanolic culturemedia and inoculums. The present work is concerned with exploring antimicrobial activity of chloroform extract and aqueous extract. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of. Scholars research library antibacterial potential of tridax. Antihyperuricemia, antioxidant, and antibacterial activities of tridax. Scholars research library antibacterial potential of. The extract of whole plant of tridax showed antibacterial activity only against. Phytochemical screening and in vitro antimicrobial activity of tridax procumbens l. Antimicrobial activity is mostly investigated by disc diffusion method and agar well diffusion method 6. Phytochemical and antibacterial studies of leaves of tridax. Evaluation of antimicrobial activity to investigate antimicrobial activity of tridax procumbens l. Synthesis of silver nanoparticles using tridax procumbens.
Jun 09, 2015 herbal products derived from hemidesmus indicus l. Later, the activities of synthesized biogenic nanoparticles were also compared with various plant extracts aqueous, ethanolic and methanolic of t. The methanolic extract of tridax procumbens showed significant antimicrobial activity against positive staphylococcus aureus and on e. Himakshi bhatikushwaha and c p malik school of life sciences, jaipur national university, jaipur 302 017, india received 14 march 20. Hence we can say the active components which are present t. The aim of the present study was to identify the bioactive compounds and to investigate the antibacterial activity from the leaves of. In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of tridax procumbens flowers extract against e. Invitro evaluation of the synergistic antimicrobial. Various extracts on waterborne bacterial pathogens uzma bathool, naziya habeeb m. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The antibacterial activity of methanolic and ethyl acetate leaf extracts of tridax procumbens linn l. Pdf on jan 10, 2011, chitra pai and others published antibacterial activity of tridax procumbens with special reference to nosocomial.
The antioxidant activity of synthesized agnps and aqueous extract of tridax procumbens leaves was determined using dpph free radical scavenging, metal chelating, and. Perform the phytochemical screening of ethanolic, methanolic and chloroform extracts of leaves, stem and flower for tridax procumbens. The activity was done by agardisc and well diffusion method 16. The methanolic extract of flowers of tridax procumbens was evaluated for their mic against e. The present study concluded that tridax procumbens can be used as a source for green synthesis of agnps which possesses potent antibacterial and antioxidant activities. Antioxidant, antidiabetic, antimicrobial and hemolytic. Antimicrobial activity of extracts of the plants was investigated by agar disc welldiffusion method against bacterial pathogens gram positive organisms. Mechanism of antibacterial action of the alcoholic. Anthocyanins are present in aqueous, methanolic, chloroform, alcoholic and ethyl acetate extracts of thespesia and totally absent in tridax. Department of chemistry, college of natural sciences, can tho university. The antibacterial activity of the plant parts extracts stem, root, leaf, flower and whole plant of tridax procumbens l. Ethyl acetate extract of this plant was separated to different fractions by column chromatography cc using chloroform and methanol as. Fresh plant juice is applied twice a day for 34 days to cure cuts and wounds. Chitra pai, ujjwala kulkarni, manjusha borderlands, sowmya murali p, mrudala yashwant deshmukh.
Assessment of antibacterial and antifungal activities of. Pdf antibacterial activity of tridax procumbens with. Keeping this view in mind, the invitro antimicrobial activity of hot water and methanolic extracts of the leaves of tridax procumbens was tested by plate diffusion. Therefore the leaves of tridax procumbens can be considered to be the promising source of antimicrobial compounds. Comparative study of antimicrobial activity of tridax. In this study, we evaluated the potential use of t. In the present study, it was planned to investigate the phytochemical and in vitro.
January antimicrobial activity of tridax procumbens against. Antibacterial activity of agnps was evaluated against reference and mdr bacterial strains. The results are represented in the figure 1, 2 and 3. Antihyperuricemia, antioxidant, and antibacterial activities. Plants are known to provide biologically active molecules with reduced or no toxicity. Comparative study of antimicrobial activity of tridax procumbens and vernonia cinerea against bacterial strains of e. Ankita jain 1, dv rao 1, amla batra 1 and amita jain 2. It also proves the traditional claim with regard to tridax procumbens. The leaf juice possesses antiseptic, insecticidal and parasiticidal properties and is used also to check haemorrhage from cuts, bruises and. Phytochemicals and antibacterial activities of leaf extract. Hence we can say the active components which are present cumbens are exbhiting antimicrobial activity from the above results. A comparative antimicrobial analysis of tridax procumbens l. Bharathi and others published antibacterial activity of tridax procumbens linn find, read and cite all the research.
Tridax procumbens linn is a tropically distributed medicinal plant. Bioactivities of extracts and isolation of compounds from tridax procumbens l. An aqueous extract was prepared similarly by using only the powder and 500 ml of sterile water. The flower plant extracts demonstrated good antimicrobial activity against these bacteria tested with inhibition zones. It has anticoagulant, antifungal, antidiarrhoel and insect repellent properties 11 12. Antibacterial activity of tridax procumbens with special. Traditionally, the stem of tridax has been very much associated with antimicrobial activity mundada and shivhare, 2010. We have synthesized silver nanoparticles by adding the 50ml of 1mm silver nitrate solution in to the plant extract and characterized by uvvis absorption spectroscopy, and sem techniques. It has hepatoprotective activity, antiinflammatory 14, and. On the basis of these activities of tridax procumbens. Phytochemical estimation and antioxidant activity were carried out for aqueous extract. Their antibacterial activities were evaluated in vitro against clinical bacterial isolates.
The antibacterial activity of tridax procumbens mediated fe3o4 nanoparticles was performed against pseudomonas aeruginosa using agar well diffusion method. List of various pharmacological activity of parts of plant tridax procumbenc linn. Antimicrobial activity of methanolic extract of flowers of. Tridax procumbens using 500ml of 80% ethanol in a soxhlet extractor for 48 hours. To the best of our knowledge, this is the first report on antibacterial activities of different phytochemical extracts and mode of action of the bacterial susceptibility compound from the leaves of t. This study aimed at evaluating the phytochemical compounds and antibacterial activities of the leaf extract of tridax procumbens. The results lend credence to the folkloric use of this plant in treating microbial infection and showed that tridax procumbens leaves and stem could be exploited for new potent antimicrobial agents. Pdf antimicrobial activity of methanolic extract of. Tanuja murab1, preeti chandurkar2, nidhi gujar3 and namita ahakey4 department of biotechnology, career college1,2,3,4, govindpura, bhopal m. Dada2 1department of microbiology, adekunle ajasin university, akungbaakoko ondo state, nigeria. The phytochemical analysis showed presence of tannins by cumbens linn and p. The tpf have been reported to increase osteogenic functioning in mesenchymal stem cells.
Fehlings solution, tridax procumbens, cu2o nanoparticles, polyaniline, li 1. A plausible antibacterial green synthesized agnps from. Oxidative stress and impaired antioxidant system have been implicated in the pathophysiology of diverse disease states. In conclusion tridax procumbens leaf extract terminates most propitious source. The mean of three replicates of zone of inhibition mm around well with tridax procumbens mediated fe3o4 nanoparticles is presented in the table 1. Plant pharmacological activity whole plant antimicrobial activity anticoagulant, hair tonic, antifungal and insect. This tridax procumbens plant was studied for its antimicrobial activity test by many researchers as alone 14. Phytochemicals and antibacterial activities of leaf. Bioactivities of extracts and isolation of compounds from. Though the extracts of these plants were found to be antimicrobial in nature and have the potential to be used in clinics, the mechanism of action of is not reported. Antibacterial evaluation of aqueous and methanol extracts. Foods free fulltext antihyperuricemia, antioxidant, and. Shows that the antimicrobial activity of ag nanoparticles from tridax plant extract.